Human papillomavirus (HPV) in Egyptian females: study by cytology, histopathology, colposcopy and molecular diagnosis of high risk types
- Author:
Mona Abdelbadiaa
;
Olfat G Shaker
;
Hala N Hosni
;
Sara E Khalifa
;
Ahmed F Shazly
- Publication Type:Journal Article
- Keywords:
HPV;
Egypt;
ISH-PCR;
pap smear;
histopathology
- From:The Malaysian Journal of Pathology
2016;38(3):257-266
- CountryMalaysia
- Language:English
-
Abstract:
Objectives: In Northern Africa, the region Egypt belongs to, about 10.7% of women are estimated to
harbour cervical human papillomavirus (HPV) infection and 78.4% of invasive cancers are attributed to
HPVs 16 or 18. We aimed at comparing HPV detection by ISH-PCR tissue with other conventional
available cheaper techniques, finding which of them can be relied upon in a developing country like
Egypt for HPV detection. Methods: Sixty patients were included. For them colposcopy, PAP smear,
histopathology and detection of HPV using ISH PCR tissue and PCR swab were achieved. Results:
PCR-ISH tissue was positive in 53.33%, 46.6% were negative. Pap smear was negative in 26 cases
(43.33%) and 43 cases (56.67%) were positive. LSIL with perinuclear halo represented nearly half of
the positive cases (16/34; 47.05%), 10 cases were diagnosed as HSIL, 4 cases as ASCUS and 4 as AGC.
Histopathology was negative in 12 (20%) cases and 48 (80%) cases were positive. CIN I and CIN I+
koliocytosis represented half of the cases (30/60) and more than half of positive cases (30/48; 62.5%).
Comparing the results of pap smear, histopathology, colposcopy and PCR swab with ISH PCR tissue,
highly significant results were seen with sensitivity of 87.5%, 100%, 62.5% and 56.2% respectively but
the specificity were 78.6%, 42.9%, 28.6% and 100% respectively. Conclusion: Conventional cytology
and histopathology were sensitive tests for detection of HPV. This may help for early detection of
cancer cervix in a developing country like Egypt. PCR swab showed the highest specificity and the
lowest sensitivity.
