Purification, expression and partial characterization of glutathione s-transferases (GSTs) from three different strains of Aedes albopictus (Diptera: Culicidae)
- Author:
Hamzah, S.N. and Alias, Z.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2016;33(2):335-347
- CountryMalaysia
- Language:English
-
Abstract:
Glutathione S-transferases (GSTs) are multifunctional enzymes involved in
detoxification of xenobiotic compounds in many insect groups. This work investigated the
expression of GSTs in Aedes albopictus by using affinity chromatography and proteomic
analysis. The study compared the expression of GSTS isoforms in larvae of three Ae. albopictus
strains (susceptible (VCRU) strain, field Kuala Lumpur (KL) strain and laboratory permethrintreated
strain). The study demonstrated that the expression of sixteen isoforms (GSTS1-1,
GSTS1-4, GSTS1-3, GSTS1-4, GSTD1-3, GSTD1-2, GSTD1-5, GSTD1-6, GSTD1-7, GSTD1-8,
GSTD1-9, GSTD1-10, GSTD1-11, GSTT, GSTD1-12, and GSTD1-13) were significantly increased
in the field KL strain (p<0.05) compared to the susceptible VCRU strain. GSTD1-1, GSTD1-2
and GSTS1-5 showed no significant difference in expression between the two strains (p>0.05).
However, while comparing the expression of GST isoforms in field KL larvae and laboratory
permethrin-treated strain, the same pattern was observed for all the GSTs especially for
Theta and Sigma class (p>0.05). In Delta class, only GSTD1-1, GSTD1-9, GSTD1-11, GSTD1-
12 and GSTD1-13 shows significant difference in expression between these two strain (p<0.05).
This comparative data on GST expression in Ae. albopictus can be useful database to identify possible underlying mechanisms governing insecticide resistance by GSTs.
- Full text:P020160901593247434492.pdf
