Detection of Mycobacterium avium subsp. Paratuberculosis in bovine faecal samples by Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) TaqMan Assay
- Author:
Narang, D., Chandra, M., Sharma, N.S.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2016;33(2):327-334
- CountryMalaysia
- Language:English
-
Abstract:
Mycobacterium avium subsp. paratuberculosis, the causative agent of Johne’s
disease is a degenerative chronic granulomatous disease of bovines. In the present study,
quantitative real time polymerase chain reaction (qRT-PCR) using TaqMan chemistry targeting
the IS900 sequence of Mycobacterium avium subsp. paratuberculosis (MAP) was employed
for the molecular diagnosis of the disease in bovine faecal samples. Out of 200 bovine faecal
samples processed, 7 samples were tested as positive by IS900 qRT-PCR. The sensitivity limit
of detection of MAP DNA in faecal samples by qRT-PCR TaqMan assay was found to be
0.05pg. No amplification was observed in other Mycobacterial spp. viz. M. phlei, M. smegmatis,
M. intracellulare and M. kansasii.
- Full text:P020160901592799468693.pdf
