Generation and characterisation of human umbilical cord derived mesenchymal stem cells by explant method
- Author:
Zuraidah Yusoff
;
Maryam Maqbool
;
Elizabeth George
;
Roshida Hassan
;
Rajesh Ramasamy
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cell;
Explant method;
Human umbilical cord;
Immunophenotyping;
Immunosuppression
- MeSH:
Mesenchymal Stromal Cells
- From:
The Medical Journal of Malaysia
2016;71(3):105-110
- CountryMalaysia
- Language:English
-
Abstract:
Mesenchymal stem cells (MSCs) derived from human
umbilical cord (UC) have been considered as an important
tool for treating various malignancies, tissue repair and
organ regeneration. Umbilical cord-derived mesenchymal
stem cells (UC-MSCs) are better alternative to MSCs that
derived from bone marrow (BM-MSCs) as they are regarded
as medical waste with little ethical concern for research and
easily culture-expanded. In this present study, the foetal
distal end of human UC was utilised to generate MSC by
explant method. Upon in vitro culture, adherent cells with
fibroblastic morphology were generated with rapid growth
kinetics. Under the respective inductive conditions, these
cells were capable of differentiating into adipocytes and
osteocytes; express an array of standard MSC’s surface
markers CD29, CD73, CD90, CD106 and MHC-class I. Further
assessment of immunosuppression activity revealed that
MSCs generated from UC had profoundly inhibited the
proliferation of mitogen-activated T lymphocytes in a dosedependent
manner. The current laboratory findings have
reinforced the application of explant method to generate UCMSCs
thus, exploring an ideal platform to fulfil the
increasing demand of MSCs for research and potential
clinical use.
- Full text:P020160808588620910493.pdf
