Staphylococcus aureus carriage in selected kindergartens in Klang Valley
- Author:
Nurul Azmawati Mohamed
;
Shalinawati Ramli
;
Nur Natasha Zulkifli Amin
;
Wan Shahida Wan Sulaiman
;
Ilina Isahak
;
Tengku Zetty Maztura Tengku Jamaluddin
;
Nooriah Mohammed Salleh
- Publication Type:Journal Article
- Keywords:
Nasal colonisation;
Staphylococcus aureus;
children
- MeSH:
Staphylococcus aureus
- From:
The Medical Journal of Malaysia
2016;71(2):62-65
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Nasal colonisation of S. aureus in healthy
children was 18% to 30%. One to three percent of them were
colonised by Methicillin-resistant Staphlycoccus aureus
(MRSA). Although MRSA infection has become increasingly
reported, population-based S. aureus and MRSA
colonisation estimates are lacking. The main objective of
this study was to determine the prevalence of S. aureus
carriage among children.
Methods: Nasal samples for S. aureus culture were obtained
from 250 children from three kindergartens in the Klang
Valley, after consent was obtained from the children and
their parents. Swabs were transported in Stuart medium,
and inoculated on mannitol-salt agar within four hours of
collection. Identification and disk diffusion test were done
according to guidelines. Polymerase chain reaction was
done on MRSA isolates for the presence of mecA and lukS/FPV
genes.
Results: Overall prevalence of S. aureus and MRSA carriage
were 19.2% (48/250) and 1.6% (4/250) respectively. mecA
gene was present in all isolates, 50% isolates carried
Panton-Valentine leucocidin (PVL) gene. Sccmec type I was
found in 2 isolates and the remaining isolates has Sccmec
type V.
Conclusion: The prevalence of S. aureus and MRSA carriage
were similar to other studies. However, risk of contracting
severe infection might be higher due to presence of PVL
gene in half of the MRSA isolates.
- Full text:P020160628393931254940.pdf
