Comparison Of The Anaerocult A And The Oil Blocking Methods For The In Vitro Cultivation Of Entamoeba Histolytica
- Author:
Nurul Azira Mohd Shah
- Publication Type:Journal Article
- Keywords:
Entamoeba histolytica;
cultivation methods
- From:The Malaysian Journal of Pathology
2015;37(3):271-274
- CountryMalaysia
- Language:English
-
Abstract:
Entamoeba histolytica, the causative agent for human amoebiasis, is among the most deadly
parasites, accounting for the second highest mortality rate among parasitic diseases. Because this
parasite dwells in low oxygen tension, for its cultivation, microaerophilic conditions are required to
mimick the human gut environment. Several methods developed for optimal growth environment are
commercially available and some are conventionally modified in-house which include the Anaerocult
A and oil blocking preparation methods. This study was undertaken to compare the reliability of the
Anaerocult A and the oil blocking methods in generating anaerobic environment for cultivation of
E. histolytica. The trophozoites of E. histolytica HM1: IMSS strains were axenically cultivated in
TYI-S-33 medium in culture incubated anaerobically by using Anaerocult A (Merck) and mineral
oil blocking method. The outcomes of both methods were determined by the minimum inhibitory
concentration (MIC) of metronidazole against E. histolytica by giving a score to the growth pattern
of the trophozoites. The reliability of both methods was assessed based on susceptibility testing of
E. histolytica to metronidazole. The MIC obtained by both anaerobic condition methods was 6.25ug/
ml, thus showing that oil-blocking method is comparable to the Anaerocult A method and therefore,
considered as a reliable method for generating an anaerobic environment for the cultivation of E.
histolytica.
- Full text:P020160315419981761297.pdf
