Molecular Detection Of The New Delhi Metallo-B-Lactamase-1 Gene In Enterobacteriaceae Isolates In A Tertiary Medical Centre
- Author:
Nor Zanariah Zainol Abidin
;
Anita Sulong
;
Hanafiah Alfizah
- Publication Type:Journal Article
- Keywords:
carbapenemase;
Enterobacteriaceae;
metallo-b-lactamase;
NDM-1
- MeSH:
Molecular Detection Of The New Delhi Metallo-B-Lactamase-1 Gene In Enterobacteriaceae Isolates In A Tertiary Medical Centre
- From:The Malaysian Journal of Pathology
2015;37(3):227-232
- CountryMalaysia
- Language:English
-
Abstract:
Background: New Delhi metallo-b-lactamase-1 (NDM-1) is a relatively recent carbapenemase
enzyme that inactivates all b-lactam antibiotics with the exception of aztreonam. This study aims
to ascertain the baseline prevalence and antibiotic susceptibility patterns of NDM-1-producing
Enterobacteriaceae in a tertiary medical center in Malaysia. Methods: Over a period of one year,
all Enterobacteriaceae isolates from all clinical specimens with reduced susceptibility to at least
one carbapenem and resistance to at least one third generation cephalosporin were subjected to
antibiotic susceptibility testing by disk diffusion and molecular detection of the NDM-1 gene by
single-target PCR followed by gel electrophoresis. Results: A total of 13,098 Enterobacteriaceae
isolates were screened and 63 (0.48%) had reduced susceptibility to at least one carbapenem. Of
this 63, 18 (29%) were NDM-1-positive. Of this 18, 16 (89.0%) were Klebsiella pneumoniae, one
(5.5%) was Escherichia coli and one (5.5%) was Klebsiella ornithinolytica. Reduced susceptibility
to at least one aminoglycoside was seen in 17 (94%) of the NDM-1-positive isolates. All 18 (100%)
had reduced susceptibility to ertapenem and were resistant to all the second and third generation
cephalosporin antibiotics tested. Conclusion: The prevalence of NDM-1-producing Enterobacteriaceae
among all the Enterobacteriaceae isolates in our institution is low (0.14%) and screening for the
NDM-1 gene is best performed using ertapenem-impregnated disks.
- Full text:P020160315412241606025.pdf
