First molecular genotyping of A302S mutation in the gamma aminobutyric acid (GABA) receptor in Aedes albopictus from Malaysia
- Author:
Low, V.L., Vinnie-Siow, W.Y., Lim, Y.A.L., Tan, T.K., Leong, C.S., Chen, C.D., Azidah A.A., Sofian-Azirun, M.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2015;32(3):554-556
- CountryMalaysia
- Language:English
-
Abstract:
Given the lack of molecular evidence in altered target-site insecticide resistance
mechanism in Aedes albopictus (Skuse) worldwide, the present study aims to detect the
presence of A302S mutation in the gene encoding the gamma aminobutyric acid receptor
resistant to dieldrin (Rdl) in Ae. albopictus for the first time from its native range of South East
Asia, namely Malaysia. World Health Organization (WHO) adult susceptibility bioassay indicated
a relatively low level of dieldrin resistance (two-fold) in Ae. albopictus from Petaling Jaya,
Selangor. However, PCR-RFLP and direct sequencing methods revealed the presence of the
A302S mutation with the predomination of heterozygous genotype (40 out of 82 individuals),
followed by the resistant genotype with 11 individuals. This study represents the first fieldevolved
instance of A302S mutation in Malaysian insect species.
- Full text:W020151207596065780431.pdf
