Genetic characterization of Toxoplasma gondii from domestic animals in central China
- Author:
Qian, W.F., Yan, W.C., Wang, T.Q., Shao, X.D., Zhai, K., Han, L.F., Lv, C.C.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2015;32(3):540-544
- CountryMalaysia
- Language:English
-
Abstract:
Toxoplasma gondii is an obligate intracellular parasite that has a remarkable
ability to infect almost all warm-blooded animals, including humans. This study was aimed to
determine the genetic characteristics of T. gondii isolates from domestic animals in Henan
Province, central China. A total of 363 DNA samples, including 208 from hilar lymph nodes of
pigs, 36 from blood samples of cats, 12 from tissues of aborted bovine fetuses and 107 from
blood samples of dams with history of abortion in Henan Province, were examined for the
presence of T. gondii by nested PCR based on B1 gene. The positive DNA samples were
further genotyped by PCR-RFLP at 11 markers, including SAG1, (3’+ 5’) SAG2, alt.SAG2,
SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico. DNA samples from 9 pigs, 5 cats, and
4 dairy cows were T. gondii B1 gene positive. Nine samples were successfully genotyped at
all genetic loci, of which 5 samples from pigs, and 2 from cats were identified as ToxoDB
genotype #9, and 2 samples from cows belonged to ToxoDB genotype #225. To our knowledge,
the present study is the second report of genetic typing of T. gondii isolates from cattle in
China, and the first report of T. gondii ToxoDB#225 from cattle.
- Full text:W020151207591935639691.pdf
