Sero-prevalence study of IgE responses to allergens from Malaysian house dust (HDM) and storage mites (SM)
- Author:
Chong, K.T., Wong, S.F., Mak, J.W., Loh, L.C., Ho, T.M.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2015;32(3):524-539
- CountryMalaysia
- Language:English
-
Abstract:
Allergens of Dermatophagoides and Blomia species are well-characterized but
not for other species. This study was conducted to determine the prevalence of allergic
sensitization to house dust (HDM) and storage mites (SM). One hundred adult subjects (aged
> 18) were recruited. The mite specific IgE of all allergic subjects were higher compared with
healthy subjetcs despite being not statistically significant except for D. farinae and G.
malaysiensis. The mean serum IgE levels against HDM and SM for allergic subjects were
significantly higher compared with those in healthy subjects. They were mainly sensitized to
Dermatophagoides farinae (35%) and Glycycometus malaysiensis (37%). Immunoblots revealed
not all allergic subjects showed positive immuno-reactivity against the mites tested. Single or
multiple bands were observed for different species. The subjects were commonly sensitized
to Group 2 (9-12 kDa), 10 (38 kDa) and 18 (40-48 kDa) allergens. Twenty-one out of 60 allergic
subjects were sensitized to either one or more species. The majority of them (71%) were
sensitized to single species. The allergic subjects were mainly sensitized to D. pteronyssinus,
followed by Tyrophagus putrecentiae and Aleuroglyphus ovatus. Seven were solely sensitized
to HDM while 10 were solely sensitized to SM. Four subjects were sensitized to both. Preadsorption
study revealed no cross-reactivity. There was difference between the prevalence
and reactivity to allergens of HDM and SM in these subjects. Both ELISA and immunoblot did
not correlate well but can complement each other in improving the detection of mite allergens
to the species level.
- Full text:W020151207589839225259.pdf
