Light microscopy and molecular identification of Sarcocystis spp. in meat producing animals in Selangor, Malaysia
- Author:
Latif, B., Kannan Kutty, M., Muslim, A., Hussaini, J., Omar, E., Heo, C.C., Rossle, N.F., Abdullah, S. Kamarudin, M.A. and Zulkarnain, M.A.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2015;32(3):444-452
- CountryMalaysia
- Language:English
-
Abstract:
One thousand and forty-five tissue samples of skeletal muscles, tongue, heart,
diaphragm and esophagus were collected from 209 animals (43 sheep, 89 goats and 77 cattle)
from an abattoir in Selangor between February and October, 2013. Each sample was divided
into three pieces with each piece measuring 2-3 mm3. Each piece was then squeezed between
two glass slides and examined microscopically at x 10 magnification for the presence of
sarcocystosis. Three positive samples from each animal species were then fixed in 10%
formalin for histological processing. Seven positive samples collected from each animal
species were preserved at -80°C or 90% ethanol for gene expression studies. Microsarcocysts
were detected in 114 (54.5%) animals by light microscopy (LM). The infection rates in sheep,
goat and cattle were 86, 61.8 and 28.6% respectively. The highest rate of infection was in the
skeletal muscles of sheep (64.9%) and goats (63.6%) and in the heart of cattle (63.6%). The
cysts were spindle to oval in shape and two stages were recognized, the peripheral metrocytes
and centrally located banana-shaped bradyzoites. 18S rRNA gene expression studies confirmed
the isolates from the sheep as S. ovicanis, goats as S. capracanis and cattle as S. bovicanis.
This, to the best of our knowledge, is the first molecular identification of an isolate of S.
ovicanis and S. capracanis in Malaysia. Further studies with electron microscopy (EM) are
required in the future to compare the features of different types of Sarcocysts spp.
- Full text:W020151207545422196953.pdf
