Evaluation of an rK39-based immunochromatographic test for the diagnosis of visceral leishmaniasis in human saliva
- Author:
da Silva, M.R.B.
;
Brandão, N.A.A.
;
Dorta, M.L.
;
Fátima Ribeiro-Dias, Costa, D.L.
;
Costa, C.H.N.
;
Oliveira, M.A.P.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2015;32(2):247-256
- CountryMalaysia
- Language:English
-
Abstract:
Visceral leishmaniasis (VL) is a tropical neglected disease endemic in 98 countries
and affects more than 58 000 individuals per year. Several serological tests are available for
VL diagnosis, including an immunochromatographic (IC) test with the rK39 antigen and finger
prick-collected blood, a rapid and low-invasive test. Here, we investigate the possibility to
use saliva as a non-invasive source of biological material for the rK39 IC test. Blood samples
from 84 patients with suspected VL were screened by the rK39 IC test, and 29 were confirmed
as being infected by a positive rK39 IC test and the presence of amastigotes on smears slides
or parasite DNA (detected using PCR-RFLP) from bone marrow aspirate. The rK39 IC test
using saliva samples was positive for 17 of the 29 confirmed VL cases (58.6%). The amount of
Leishmania-specific IgG or total IgG, as evaluated by an immunoenzymatic assay, was
higher in the saliva of patients who had rK39 IC test positivity using saliva, whereas the
amount of Leishmania-specific IgA or total IgA was similar to the healthy donors. These
results suggest that saliva is not an appropriated material for diagnosing VL with this test.
- Full text:W020150708548795014072.pdf
