Molecular survey and sequence analysis of Anaplasma spp. in cattle and ticks in a Malaysian farm
- Author:
Tay, S.T.
;
Koh, F.X.
;
Kho, K.L.
;
Ong, B.L.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2014;31(4):769-776
- CountryMalaysia
- Language:English
-
Abstract:
This study was conducted to determine the occurrence of Anaplasma spp. in the
blood samples of cattle, goats, deer and ticks in a Malaysian farm. Using polymerase chain
reaction (PCR) and sequencing approach, Anaplasma spp. was detected from 81(84.4%) of
96 cattle blood samples. All blood samples from 23 goats and 22 deer tested were negative.
Based on the analysis of the Anaplasma partial 16S ribosomal RNA gene, four sequence types
(genotypes 1 to 4) were identified in this study. Genotypes 1-3 showed high sequence similarity
to those of Anaplasma platys/ Anaplasma phagocytophilum, whilst genotype 4 was identical
to those of Anaplasma marginale/ Anaplasma centrale/ Anaplasma ovis. Anaplasma DNA
was detected from six (5.5%) of 109 ticks which were identified as Rhipicephalus (formely
known as Boophilus) microplus ticks collected from the cattle. This study reported for the
first time the detection of four Anaplasma sequence types circulating in the cattle population
in a farm in Malaysia. The detection of Anaplasma DNA in R. microplus ticks in this study
provides evidence that the ticks are one of the potential vectors for transmission of
anaplasmosis in the cattle.
- Full text:W020150602325236244902.pdf
