Evaluation of codon optimized recombinant Plasmodium knowlesi Merozoite Surface Protein-119 (pkMSP-119) expressed in Pichia pastoris
- Author:
Lau, Y.L.
;
Cheong, F.W.
;
Chin, L.C.
;
Mahmud, R.
;
Chen, Y.
;
Fong, M.Y.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2014;31(4):749-759
- CountryMalaysia
- Language:English
-
Abstract:
Malaria causes high global mortality and morbidity annually. Plasmodium knowlesi
has been recognised as the fifth human Plasmodium sp. and its infection is widely distributed
in Southeast Asia. Merozoite surface protein-119 (MSP-119) appears as a potential candidate
for malaria blood stage vaccine as it could induce protective immunity. In this study, codon
optimized P. knowlesi MSP-119 (pkMSP-119) was expressed and purified in yeast Pichia pastoris
expression system. The purified recombinant protein was further evaluated using Western
blot assay using knowlesi malaria, non-knowlesi human malaria, non-malarial parasitic
infections and healthy serum samples (n = 50). The sensitivity of purified pkMSP-119 towards
detection of knowlesi infection was as 28.6% (2/7). pkMSP-119 did not react with all nonmalarial
parasitic infections and healthy donor sera, yet reacted with some non-knowlesi
human malaria sera, therefore lead to a specificity of 86.0% (37/43).
- Full text:W020150602321737495128.pdf
