Cytogenetic, crossing and molecular evidence of two cytological forms of Anopheles argyropus and three cytological forms of Anopheles pursati (Diptera: Culicidae) in Thailand
- Author:
Thongsahuan, S.
;
Otsuka, Y.
;
Baimai, V.
;
Saeung, A.
;
Hempolchom, C.
;
Taai, K.
;
Srisuka, W.
;
Dedkhad, W.
;
Sor-suwan, S.
;
Choochote, W.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2014;31(4):641-653
- CountryMalaysia
- Language:English
-
Abstract:
Nine and 11 isolines of Anopheles argyropus and Anopheles pursati, respectively,
were established from individual females collected from cow-baited traps, and the
characteristics of metaphase chromosomes were investigated in their F1-progenies. As
determined by the different amounts of extra heterochromatin on sex chromosomes, 2 types
of X (X1, X2) and Y (Y1,Y2), and 2 types of X (X1, X2) and 3 types of Y (Y1, Y2, Y3) chromosomes
were obtained from An. argyropus and An. pursati, respectively. These types of sex
chromosomes comprised 2 [Forms A (X1, Y1) and B (X1, X2, Y2)] and 3 [Forms A (X1, X2, Y1), B
(X1, X2, Y2) and C (X2, Y3)] karyotypic forms of An. argyropus and An. pursati, respectively.
All karyotypic forms acquired from An. pursati are new one that were discovered in this
study, of which Forms A, B and C were found generally in Chiang Mai Province, while only 1
isoline of Form B was obtained in Ratchaburi Province. Form A was recovered from An.
argyropus only in Ubon Ratchathani Province, whereas Form B from that species was found
commonly in both Ubon Rathchathani and Nakhon Si Thammarat Provinces. Crossing
experiments among the 2 and 3 isolines representing 2 and 3 karyotypic forms of An. argyropus
and An. pursati, respectively, indicated genetic compatibility in yielding viable progenies
and synaptic salivary gland polytene chromosomes through F2-generations. The conspecific
natures of these karyotypic forms in both species were further supported by very low
intraspecific sequence variations (average genetic distance: An. argyropus = 0.003-0.007,
An. pursati = 0-0.005) of ribosomal DNA (ITS2) and mitochondrial DNA (COI and COII).
- Full text:W020150528589162025297.pdf
