An investigation on the diversity of mosquitocidal bacteria and its relationship with incidence of vector borne diseases
- Author:
Subbiah Poopathi
;
De Britto, R.L.J
;
Thirugnanasambantham, K
;
Ragul, K
;
Mani, C
;
Balagangadharan, K.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2015;32(1):84-97
- CountryMalaysia
- Language:English
-
Abstract:
Control of mosquitoes is the most important aspect of public health, as mosquitoes
transmit many human diseases, including the fatal infection, Japanese encephalitis. This
paper addresses the isolation of new mosquitocidal bacteria from soil samples in the Union
Territory of Pondicherry, India, where, no clinical cases of vector borne infections have been
reported. Bacterial isolates from soil samples were screened for potential mosquitocidal
strains and bioassays against mosquito vectors (Culex quinquefasciatus, Anopheles stephensi
and Aedes aegypti) were carried out. Genomic DNA of potential mosquitocidal isolates was
amplified and species identification was carried out using BLASTn program (NCBI).
Phylogenetic analysis of 16S rRNA sequences of mosquitocidal bacteria revealed seven
potential isolates. SDS-PAGE results have shown that there was considerable difference in
the protein profiles. Numerical analysis revealed 4 distinct groups at similarity level 25%. The
relationship between VBDs and prevalence of soil mosquitocidal bacteria in the study sites
has elicited considerable interest in the diversity of mosquitocidal bacteria and their application
for mosquito borne diseases control.
- Full text:W020150519527990316194.pdf
