Inhibition of secretary PLA2 – VRV-PL-VIIIa of Russell‘s viper venom by standard aqueous stem bark extract of Mangifera indica L.
- Author:
Dhananjaya, B.L
;
Sudarshan, S.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2015;32(1):24-35
- CountryMalaysia
- Language:English
-
Abstract:
The aqueous extract of Mangifera indica is known to possess anti-snake venom
activities. However, its inhibitory potency and mechanism of action on multi-toxic
phospholipases A2s, which are the most toxic and lethal component of snake venom is still
unknown. Therefore, this study was carried out to evaluate the modulatory effect of standard
aqueous bark extract of M. indica on VRV-PL-VIIIa of Indian Russells viper venom. Mangifera
indica extract dose dependently inhibited the GIIB sPLA2 (VRV-PL-VIIIa) activity with an IC50
value of 6.8±0.3 μg/ml. M. indica extract effectively inhibited the indirect hemolytic activity
up to 96% at ~40 μg/ml concentration. Further, M. indica extract at different concentrations
(0-50 μg/ml) inhibited the edema formed in a dose dependent manner. It was found that there
was no relieve of inhibitory effect of the extract when examined as a function of increased
substrate and calcium concentration. The inhibition was irreversible as evident from binding
studies. The in vitro inhibition is well correlated with in situ and in vivo edema inducing
activities. As the inhibition is independent of substrate, calcium concentration and was
irreversible, it can be concluded that M. indica extracts mode of inhibition could be due to
direct interaction of components present in the extract with PLA2 enzyme. In conclusion, the
aqueous extract of M. indica effectively inhibits svPLA2 (Snake venom phospholipase A2)
enzymatic and its associated toxic activities, which substantiate its anti-snake venom
properties. Further in-depth studies are interesting to known on the role and mechanism of
the principal inhibitory constituents present in the extract, so as to develop them into potent
anti-snake venom and as an anti-inflammatory agent.
- Full text:W020150519506420462799.pdf
