Evaluation of DNA and RNA Extraction Methods
- Author:
C S Edwin Shiaw
;
M S Shiran
;
Y K Cheah
;
G C Tan
;
A R Sabariah
- Publication Type:Journal Article
- Keywords:
FFPE, DNA extraction, RNA extraction, crosslinking
- From:
The Medical Journal of Malaysia
2010;65(2):133-137
- CountryMalaysia
- Language:English
-
Abstract:
This study was done to evaluate various DNA and RNA
extractions from archival FFPE tissues. A total of 30 FFPE
blocks from the years of 2004 to 2006 were assessed with
each modified and adapted method. Extraction protocols
evaluated include the modified enzymatic extraction
method (Method A), Chelex-100 extraction method (Method
B), heat-induced retrieval in alkaline solution extraction
method (Methods C and D) and one commercial FFPE DNA
Extraction kit (Qiagen, Crawley, UK). For RNA extraction, 2
extraction protocols were evaluated including the
enzymatic extraction method (Method 1), and Chelex-100
RNA extraction method (Method 2). Results show that the
modified enzymatic extraction method (Method A) is an
efficient DNA extraction protocol, while for RNA extraction, the enzymatic method (Method 1) and the Chelex-100 RNA extraction method (Method 2) are equally efficient RNA extraction protocols.
