Broad reactive monoclonal antibodies for rapid identification of enteroviruses show cross-reactivity with chikungunya virus infected cells
- Author:
Khairul AH
;
Chem YK
;
Keniscope C
;
Rosli J
;
Hassan S
;
Mat Jais MS
;
Chua KB
- Publication Type:Journal Article
- Keywords:
enterovirus 71, chikungunya virus, cytopathic effect, Vero cells, cross-reaction, viral diagnosis
- From:The Malaysian Journal of Pathology
2010;32(1):49-52
- CountryMalaysia
- Language:English
-
Abstract:
In the past decade, enterovirus 71 (EV71) and chikungunya (CHIK) virus have re-emerged periodically
causing serious public health problems in Malaysia, since their first emergence in 1997 and 1998
respectively. This study demonstrates that CHIK virus causes similar patterns of cytopathic effect
in cultured Vero cells as some enteroviruses. They also show positive cross-reaction on direct
immunofl uorescence staining using monoclonal antibodies meant for typing enteroviruses. Without
adequate clinical and epidemiological information for correlation, CHIK virus isolated from patients
with acute febrile rash can be wrongly reported as untypeable enterovirus due to its cross-reactivity
with commercial pan-enterovirus monoclonal antibodies. This is due to the diagnostic laboratory being
unaware of such cross-reactions as it has not been reported previously. Final identifi cation of the
virus could be determined with specific antibodies or molecular typing using specifi c oligonucleotide
primers for the CHIK virus.
- Full text:W020151209335603281015.pdf
