Mixed-genotypes Infections with Hepatitis C Virus in Hemodialysis Subjects
- Author:
Hairul Aini H
;
Mustafa M I A
;
Seman M R
;
Nasuruddin B A
- Publication Type:Journal Article
- Keywords:
Hemodialysis, hepatitis C virus, mixed-genotype infection
- From:
The Medical Journal of Malaysia
2012;67(2):199-203
- CountryMalaysia
- Language:English
-
Abstract:
Mixed-genotypes hepatitis C virus (HCV) infections are
normally ignored in chronic hemodialysis patients. The aim
of this study is to investigate the prevalence of mixedgenotypes infections among hemodialysis patients in Pahang province, Malaysia. Reverse-transcription and polymerase chain reaction methods were performed using two different sets of primers, targeting the 5’ untranslated region and nonstructural 5B region. Target region base sequences were obtained by direct sequencing. Discrepancy in outcomes from phylogenetic analysis of both regions suggests double infections. Of 40 subjects in eight hemodialysis centres, evidence of mixed-genotypes infections was found in 5 subjects (12.5%) from three different centres. Four patients were infected with mixed genotypes 3 and 1 and one with genotypes 3 and 4. Cases of mixed HCV genotypes infection were considered high among hemodialysis patients in Pahang. However, further investigation is needed to confirm
whether they are true mixed infections or perhaps infection
with recombinant virus and also to assess the
clinicopathologic characteristics of the infection.
