A novel cost-effective medium for the production of Bacillus thuringiensis subsp. israelensis for mosquito control
- Author:
Subbiah Poopathi
;
Archana, B.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2012;29(1):81-91
- CountryMalaysia
- Language:English
-
Abstract:
Abstract. Bacillus thuringiensis subsp. israelensis (Bti) has been used for mosquito-control
programmes the world-wide. Indeed, the large-scale production of Bti for mosquito control is
very expensive due to the high cost of its culture. In the present study, we attempted to widen
the scope in developing cost-effective culture medium for Bti production, based on the raw
materials available on the biosphere, including coconut cake powder, CCP (Cocos nucifera),
neem cake powder, NCP (Azadirachta indica) and groundnut cake powder, GCP (Arachis
hypogea). Among these raw materials, the biomass production of Bti, sporulation and toxin
synthesizing from ‘CCP’ in combination with mineral salt (MnCl2) was comfortably satisfactory.
Bioassays with mosquito species (Culex quinquefasciatus, Anopheles stephensi and Aedes
aegypti) and field trials were also satisfactory. The present investigation suggests that coconut
cake-based culture medium can be used as an alternative for industrial production of Bti in
mosquito-control programme. Therefore, the study is very important from the point of effective
production of Bti from cost-effective culture medium for the control of mosquito vectors.
- Full text:W020150618348078067008.pdf
