The Harmful Effects of Superoxide Radical and the Protective Effect of Ginseng Saponin on Rat Whole Bladder Contractility.
- Author:
Hyung Jee KIM
1
;
Jae Hong PARK
;
Gil Ho LEE
;
Kwan joong JOO
Author Information
1. Department of Urology, Dankook University College of Medicine, Cheonan, Korea. killtumor@yahoo.co.kr
- Publication Type:Original Article
- Keywords:
Superoxide radical;
Bladder contractility;
Ginseng saponin
- MeSH:
Animals;
Baths;
Ditiocarb;
NG-Nitroarginine Methyl Ester;
Nitroprusside;
Panax*;
Rats*;
Saponins*;
Superoxides*;
Transducers;
Urinary Bladder*
- From:Korean Journal of Urology
2002;43(6):496-501
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We investigated the effects of the superoxide radical on rat whole bladder contractility with duroquinone (superoxide radical generator, Dq) and diethyldithiocarbamate (superoxide dismutase inhibitor, DETCA), and the effects of ginseng saponin (GS) against superoxide radical injury. MATERIALS AND METHODS: Isometric tension changes of isolated rat whole bladders were recorded in an organ bath using a force transducer. The acute effects of Dq and Dq preincubated with DETCA were assessed on resting tension, electrical field stimulation, and bethanechol-, ATP-, and KCl-induced contraction. The effects of Dq and Dq preincubated with DETCA in the presence of sodium nitroprusside and GS were investigated. RESULTS: The resting tension of the muscle was not changed by Dq and Dq preincubated with DETCA. Dq had a harmful effect on only ATP- and KCl-induced detrusor contraction, whereas Dq pretreated with DETCA attenuated the induction of detrusor contraction which was reduced in response to the exogenous NO including GS. In the presence of L-NAME, the effects of GS reduced the Dq-induced inhibition on the detrusor contractility. CONCLUSIONS: It is suggested that the superoxide radical may be the cause of voiding difficulty. GS, as a NO synthesis stimulator, seems to act as a scavenger of the superoxide anion. However further study on the effect of each subfraction of GS is needed for clinical application.
