Adequacy of cellular material in split-sampling of cervical scrapings for routine cancer screening: an analysis of 702 smears
- Author:
Norodiyah Othman
;
Nor Hayati Othman
- Publication Type:Journal Article
- Keywords:
Split-sampling, primary cytology, ThinPrep® cytology, discarded sample
- From:The Malaysian Journal of Pathology
2012;34(2):115-121
- CountryMalaysia
- Language:English
-
Abstract:
Objective: The aim of this study was to examine cells (split-sample) that were retained on sampling
devices used to collect conventional Pap smears (primary smears) in order to evaluate specimen
adequacy and cytological diagnosis of scrapings that are routinely discarded. Study design: Cervical
scrapings from women attending routine cervical cancer screening were obtained using a cervical
brush. Following primary conventional smear preparation, the same sampling devices were rinsed
in Preservcyt solution (Cytyc) for subsequent monolayered thin smear (split-sample/discarded
sample). The smears (conventional and ThinPrep® monolayer) were examined independently by
pathologists and classifi ed using the Bethesda System. The diagnoses from discarded samples (splitsample
smears) were then compared with the diagnoses made on primary conventional Pap smears.
Results: 702 samples were studied. Cell abnormalities was found in 14/702 conventional smear and
12/702 split-sample thin smear. The adequacy of sampling in primary smears was 94.7% compared
to 88.9% in split-sample smears. Six cases of Human Papillomavirus infection was found in splitsample
smear, whereas only 5 cases found in primary smear. Cohen’s Kappa was 0.61 showing
substantial agreement between both sampling cytological results. Conclusion: The cervical brush
discarded after conventional smear retain adequate number of cells for diagnostic purposes.
- Full text:W020151218568868724532.pdf
