Real-time quantifi cation for BCR-ABL transcripts in chronic myeloid leukaemia patients in UKMMC, Malaysia
- Author:
FL Wong
;
NH Hamidah
;
AA Hawa
;
AN Nurul
;
CF Leong
;
SAW Fadilah
;
O Ainoon
- Publication Type:Journal Article
- Keywords:
CML, BCR-ABL, RQ-PCR
- From:The Malaysian Journal of Pathology
2011;33(2):107-112
- CountryMalaysia
- Language:English
-
Abstract:
Molecular pathogenesis of chronic myeloid leukemia (CML) is well established and molecular
monitoring for patients with CML has become an important practice in the management of patients
on imatinib therapy. In the present study, we report the use of RQ-PCR method for detection of
BCR-ABL fusion gene for our CML cases. We performed a two-step RQ-PCR on bone marrow
aspirates or peripheral blood of 37 CML patients. Quantitative expression of BCR-ABL fusion
gene was carried out relative to the expression of a housekeeping gene as endogenous control to
compensate for uneven cell numbers, RNA quality, or variations in reverse transcription effi ciencies.
Twenty-four of these patients were pre-treated with hydroxyurea or alpha interferon prior to the
imatinib therapy. Their BCR-ABL fusion gene levels were monitored for 18 months. All samples
processed were evaluable. The PCR amplifi cation effi ciency of the ABL gene is 90.5% (0.2158)
and the BCR-ABL gene, 93.4% (0.1573).
