CD133 marks for colorectal adenocarcinoma
- Author:
Man-Fong Chew
;
Kean-Hooi Teoh
;
Phaik-Leng Cheah
- Publication Type:Journal Article
- Keywords:
Colorectal adenocarcinoma, CD133, immunohistochemistry
- From:The Malaysian Journal of Pathology
2012;34(1):25-28
- CountryMalaysia
- Language:English
-
Abstract:
CD133, a marker which has been advocated to mark colorectal carcinoma “stem or tumour initiating
cells” is amongst the frequently studied markers in colorectal cancer. A study was conducted at
the Department of Pathology, University of Malaya Medical Centre to determine the expression of CD133 in 56 archived, formalin-fi xed, paraffi n-embedded colorectal adenocarcinoma in comparison with adjacent benign colorectal epithelium by immunohistochemical staining for CD133 expression. CD133 immunopositivity was determined as staining at the glandular luminal surface or in the
intraluminal debris. Expression was semiquantitated for (1) proportion of CD133 immunopositivity in the malignant or adjacent benign colorectal epithelium and (2) intensity of staining. The fi nal score of CD133 immunopositivity was arbitrarily taken as proportion of CD133 immunopositivity multiplied by intensity of staining in both the malignant and adjacent benign colorectal epithelium.CD133 expression was observed in signifi cantly increased frequency in 49 (87.5%) colorectal
adenocarcinoma compared with 15 (26.8%) of the adjacent benign colorectal epithelium (p<0.05). In terms of immunopositivity score (proportion of CD133 immunopositivity multiplied by intensity of staining), colorectal adenocarcinoma had a mean arbitrary score of 8.5 which was signifi cantly higher than the mean immunopositivity score of 0.5 of the adjacent benign colorectal epithelium (p<0.05). In addition, the maximum immunopositivity score for the adjacent benign colorectal
epithelium was 4, while 38 (67.9%) of colorectal adenocarcinoma had scores >4. This study shows
that CD133 is able to mark colorectal adenocarcinoma but it is still unclear at this juncture whether
CD133 is indeed a marker for colorectal adenocarcinoma “stem cells”.
- Full text:W020151218423640917428.pdf
