Reduced susceptibility of Malaysian clinical isolates of Burkholderia pseudomallei to ciprofloxacin
- Author:
Liew, F.Y.
;
Tay, S.T.
;
Puthucheary, S.D.*
- Publication Type:Journal Article
- From:Tropical Biomedicine
2011;28(3):646-650
- CountryMalaysia
- Language:English
-
Abstract:
Ciprofloxacin, a quinolone with good intracellular penetration may possibly be
used for treatment of melioidosis caused by Burkholderia pseudomallei, but problems with
resistance may be encountered. Amino acid substitutions in gyrA/gyrB have given rise to
fluoroquinolone resistance in various microorganisms. Using published primers for gyrA and
gyrB, PCR was performed on 11 isolates of B. pseudomallei with varying degrees of sensitivity
to ciprofloxacin, followed by DNA sequencing to detect possible mutations. Results showed
an absence of any point mutation in either gene. Local isolates have yet to develop full
resistance to ciprofloxacin and probably other mechanisms of resistance may have been
involved in the decreased sensitivity to ciprofloxacin.
- Full text:W020150615420760432764.pdf
