Application of amplified ribosomal DNA restriction analysis in identification of Acinetobacter baumannii from a Tertiary Teaching Hospital, Malaysia
- Author:
Kong, B.H.,
;
Hanifah, Y.A.
;
Yusof, M.Y.
;
Thong, K.L.,
- Publication Type:Journal Article
- From:Tropical Biomedicine
2011;28(3):563-568
- CountryMalaysia
- Language:English
-
Abstract:
Acinetobacter baumannii, genomic species 3 and 13TU are being increasingly
reported as the most important Acinetobacter species that cause infections in hospitalized
patients. These Acinetobacter species are grouped in the Acinetobacter calcoaceticus-
Acinetobacter baumannii (Acb) complex. Differentiation of the species in the Acb-complex
is limited by phenotypic methods. Therefore, in this study, amplified ribosomal DNA restriction
analysis (ARDRA) was applied to confirm the identity A. baumannii strains as well as to
differentiate between the subspecies. One hundred and eighty-five strains from Intensive
Care Unit, Universiti Malaya Medical Center (UMMC) were successfully identified as A.
baumannii by ARDRA. Acinetobacter genomic species 13TU and 15TU were identified in 3
and 1 strains, respectively. ARDRA provides an accurate, rapid and definitive approach
towards the identification of the species level in the genus Acinetobacter. This paper reports
the first application ARDRA in genospecies identification of Acinetobacter in Malaysia.
- Full text:W020150615389052783547.pdf
