Sequence analysis on the mitochondrial COXI gene of recent clinical isolates of Plasmodium knowlesi in Klang valley, peninsular Malaysia
- Author:
Mun Yik Fong*
;
Yee-Ling Lau
;
Lit-Chien Chin
;
Abdulsalam M.Q. Al-Mekhlafi
- Publication Type:Journal Article
- From:Tropical Biomedicine
2011;28(2):457-463
- CountryMalaysia
- Language:English
-
Abstract:
The cytochrome oxidase subunit I (COXI) gene sequences of three recent (2007-
2008) clinical Plasmodium knowlesi isolates from Klang Valley, peninsular Malaysia, were
determined and compared with those of older (1960’s) peninsular Malaysia, recent isolates
from Sarawak (on Borneo Island), and an isolate from Thailand. Multiple alignment of the
sequences showed that the three clinical isolates were more similar to the older peninsular
Malaysia isolates than to those from Sarawak and Thailand. Phylogenetic tree based on the
COXI sequences revealed three distinct clusters of P. knowlesi. The first cluster consisted of
isolates from peninsular Malaysia, the second consisted of Sarawak isolates and the third
composed of the Thailand isolate. The findings of this study highlight the usefulness of
mitochondrial COXI gene as a suitable marker for phylogeographic studies of P. knowlesi.
- Full text:W020150615382094039990.pdf
