In vitro cultivation of axenic amastigotes and the comparison of antioxidant enzymes at different stages of Leishmania tropica
- Author:
Bahrami, S.*
;
Hatam, G.R.
;
Razavi, M.
;
Nazifi, S.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2011;28(2):411-417
- CountryMalaysia
- Language:English
-
Abstract:
The present study aimed to establish a simple method to yield large amounts of
Leishmania tropica amastigote-like forms in axenic cultures and to compare the superoxide
dismutase (SOD) and glutathione peroxidase (GPX) enzymes at different stages of L. tropica.
Different culture conditions were tested to find the optimum condition of axenic amastigotes
generation. Superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities were
determined at logarithmic and stationary phases and axenic amastigote stage of the parasite.
A high proportion (88%) of amastigote-like forms of L. tropica was observed in BHI medium
supplemented with 20% FCS, pH 4.5, and incubated at 37ºC with 5% CO2. The results showed
that SOD activity was at the lowest level in the logarithmic phase of promastigotes and
increased towards the stationary phase of promastigotes and amastigote stage. The results
showed that the optimum condition for differentiation of L. tropica promastigotes to axenic
amastigotes was BHI medium containing 20% FCS at pH 4.5, incubated at 37ºC in the presence
of 5% CO2. It seems that SOD, but not GPX is a major determinant of intracellular survival of
the parasite.
- Full text:W020150615377117782301.pdf
