Identification of local clinical Candida isolates using CHROMagar CandidaTM as a primary identification method for various Candida species
- Author:
Madhavan, P.*
;
Jamal, F.
;
Chong, P.P.
;
Ng, K.P.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2011;28(2):269-274
- CountryMalaysia
- Language:English
-
Abstract:
The objective of our study was to study the effectiveness of CHROMagar CandidaTM
as the primary identification method for various clinical Candida isolates, other than the
three suggested species by the manufacturer. We studied 34 clinical isolates which were
isolated from patients in a local teaching hospital and 7 ATCC strains. These strains were first
cultured in Sabouraud dextrose broth (SDB) for 36 hours at 35ºC, then on CHROMagar plates
at 30ºC, 35ºC and 37ºC. The sensitivity of this agar to identify Candida albicans, Candida
dubliniensis, Candida tropicalis, Candida glabrata, Candida rugosa, Candida krusei and
Candida parapsilosis ranged between 25 and 100% at 30ºC, 14% and 100% at 35ºC, 56% and
100% at 37ºC. The specificity of this agar was 100% at 30ºC, between 97% and 100% at 35ºC,
92% and 100% at 37ºC. The efficiency of this agar ranged between 88 and 100% at 30ºC, 83%
and 100% at 35ºC, 88% and 100% at 37ºC. Each species also gave rise to a variety of colony
colours ranging from pink to green to blue of different colony characteristics. Therefore, the
chromogenic agar was found to be useful in our study for identifying clinical Candida isolates
- Full text:W020150615364144815001.pdf
