Antigenic profile of Blomia tropicalis, Aleuroglyphus ovatus and Glycycometus malaysiensis
- Author:
Tang, J.C.
;
Wong, S.F.*
;
Mak, J.W.
;
Ho, T.M.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2011;28(2):223-236
- CountryMalaysia
- Language:English
-
Abstract:
House dust mites and storage mites are well-known causes for allergenic diseases.
The aim of this study was to investigate the immunogenic sites of Blomia tropicalis,
Aleurogyphus ovatus and Glycycometus malaysiensis. The mites were maintained in a
culture medium at 25ºC and 75% relative humidity. Mites were harvested either with heat
escape or floatation method, purified, homogenized, quantified and used for the production of
polyclonal antibody and immunostaining. For each species of mites, five male mice and five
male rats were randomly selected and immunized intraperitoneally with respective crude
mite extract at two-weekly intervals. Blomia tropicalis, A. ovatus or G. malaysiensis whole
mites and paraffin-embedded mite sections were immunostained with the respective polyclonal
antibody. The faecal pellets of mites were intensely stained for all the three species in the
present study. The legs of sectioned A. ovatus were not immunogenic as compared with
those of G. malaysiensis and B. tropicalis. The outer layer (cuticle) of whole mites and the
eggs for these species were very immunogenic. Hence, the polyclonal antibodies obtained in
this study may serve as potential tools in detecting the eggs and immature mites in
environmental samples. Future studies should focus on the antigenic components of eggs
since they were relatively abundant in dust and highly antigenic as seen in the present study.
- Full text:W020150615356251060685.pdf
