A comparative evaluation of dengue diagnostic tests based on single acute serum samples for laboratory confi rmation of acute dengue
- Author:
CHUA KB
;
MUSTAFA B
;
ABDUL WAHAB AH
;
CHEM YK
;
KHAIRUL AH
;
KUMARASAMY V
;
MARIAM M
;
NURHASMIMI H
;
ABDUL RASID K
- Publication Type:Journal Article
- Keywords:
Dengue, virus isolation, molecular diagnostics, NS1 antigen-capture ELISA
- From:The Malaysian Journal of Pathology
2011;33(1):13-20
- CountryMalaysia
- Language:English
-
Abstract:
A prospective study was carried out to evaluate the sensitivity of dengue NS1 antigen-capture
ELISA in comparison with dengue virus isolation, conventional RT-PCR and real-time RT-PCR for
laboratory confi rmation of acute dengue based on single-acute serum samples. Four primary healthcare
centres were involved to recruit patients with clinical diagnosis of dengue illness. Patient’s
demographic, epidemiological and clinical information were collected on a standardized data entry
form and 5 ml of venous blood was collected upon consent. In the laboratory, six types of laboratory
tests were performed on each of the collected acute serum sample.
Of the 558 acute serum samples collected from 558 patients with clinical diagnosis of dengue
from mid-August 2006 to March 2009, 174 serum samples were tested positive by the dengue NS1
antigen-capture ELISA, 77 by virus isolation, 92 by RT-PCR and 112 by real-time RT-PCR. A total
of 190 serum samples were tested positive by either one or a combination of the four methods
whereas, only 59 serum samples were tested positive by all four methods. Thus, based on singleacute
serum samples, 190 of the 558 patients (34.1%) were laboratory-confi rmed acute dengue. The
overall test sensitivity was 91.6%, 40.5%, 48.4% and 58.9% for dengue NS1 antigen-capture ELISA,
virus isolation, conventional RT-PCR and real-time RT-PCR respectively. Statistically, dengue NS1
antigen-capture ELISA was the most sensitive and virus isolation was the least sensitive test for the
laboratory confi rmation of acute dengue based on single-acute serum specimens. Real-time RT-PCR
was signifi cantly more sensitive than the conventional RT-PCR.
- Full text:W020151209482387816164.pdf
