Molecular analysis of 2009 pandemic Infl uenza A(H1N1) in Malaysia associated with mild and severe infections
- Author:
Pauline BALRAJ
;
Huraizah SIDEK
;
Jeyanthi SUPPIAH
;
Alan Soo Beng KHOO
;
Zainah SAAT
- Publication Type:Journal Article
- Keywords:
pandemic infl uenza virus A (H1N1), haemagglutinin, neuraminidase, variation
- From:The Malaysian Journal of Pathology
2011;33(1):7-12
- CountryMalaysia
- Language:English
-
Abstract:
The 2009 pandemic infl uenza A(H1N1) was fi rst detected in Malaysia in May 2009. It quickly
spread in the general population and contributed to a number of infl uenza-like illness. The objective
of the study is to characterize genetic changes in early Malaysian isolates of mild and severe illness
of the novel infl uenza, and to compare sequences of viruses circulating in Malaysia to those in other
countries between May to September 2009. Viral isolates of 56 mild cases and 10 severe (intensive
care unit or fatal) cases were sequenced for haemagglutinin (HA) and neuraminidase (NA). Genome
sequencing of the viral RNA was conducted on 5 isolates (3 were from fatal cases). Highly conserved
sequences with few sporadic variations were identifi ed in HA and NA. E374K and D222N were
identifi ed in 2 viral isolates from patients with severe illness. Phylogenetic analysis showed close
genetic relatedness to the vaccine strain A/California/07/09 and other isolates circulating worldwide
during the same period. Sporadic variations were identifi ed in the viral isolates, however a larger
sample size is required to make associations with disease severity.
- Full text:W020151209481730785502.pdf
