Gene expression characteristic in human auricular cartilage tissue engineering.
- Author:
Farah Wahida I
;
Aminuddin BS
;
Munirah S
;
Chua KH
;
Fuzina NH
;
Isa MR
;
Saim L
;
Ruszymah BH
- Publication Type:Journal Article
- MeSH:
Actins/genetics;
Cartilage/transplantation;
Cell Aging/physiology;
Cells, Cultured;
Chondrocytes/*cytology;
Collagen Type I/*genetics;
Collagen Type II/*genetics;
Ear, External;
Fibroblasts/cytology;
Gene Expression/physiology;
Mice, Nude;
*Phenotype;
Reverse Transcriptase Polymerase Chain Reaction;
Tissue Engineering/*methods
- From:
The Medical Journal of Malaysia
2004;59 Suppl B():190-191
- CountryMalaysia
- Language:Malay
-
Abstract:
This study was to assess collagen type II and collagen type I gene expression in tissue-engineered human auricular: cartilage formed via tissue engineering technique. Large-scale culture expansions were transformed into 3D in vitro construct and were implanted subcutaneously on the dorsal of athymic mice. After 8 weeks, explanted construct was processed in the same manner of native cartilage to facilitate cells for gene expression analysis. Isolated cells from in vivo construct demonstrated expression of type II collagen gene comparable to native cartilage. This study verified that tissue-engineered auricular cartilage expressed cartilage specific gene, collagen type II after in vivo maturation.
