Comparison of chitosan scaffold and chitosan-collagen scaffold: a preliminary study.
- Author:
Norazril SA
;
Aminuddin BS
;
Norhayati MM
;
Mazlyzam AL
;
Fauziah O
;
Ruszymah BH
- Publication Type:Journal Article
- MeSH:
Cell Adhesion/*physiology;
*Chitosan;
*Collagen;
Energy Metabolism/physiology;
Fibroblasts/cytology;
Microscopy, Electron, Scanning;
Organ Culture Techniques/*methods;
*Polymers;
Tissue Engineering/*methods
- From:
The Medical Journal of Malaysia
2004;59 Suppl B():186-187
- CountryMalaysia
- Language:Malay
-
Abstract:
Chitosan has similar structure to glycosaminoglycans in the tissue, thus may be a good candidates as tissue engineering scaffold. However, to improve their cell attachment ability, we try to incorporate this natural polymer with collagen by combining it via cross-linking process. In this preliminary study we evaluate the cell attachment ability of chitosan-collagen scaffold versus chitosan scaffold alone. Chitosan and collagen were dissolved in 1% acetic acid and then were frozen for 24 hours before the lyophilizing process. Human skin fibroblasts were seeded into both scaffold and were cultured in F12: DMEM (1:1). Metabolic activity assay were used to evaluate cell attachment ability of scaffold for a period of 1, 3, 7 and 14 days. Scanning electron micrographs shows good cell morphology on chitosan-collagen hybrid scaffold. In conclusion, the incorporation of collagen to chitosan will enhance its cell attachment ability and will be a potential scaffold in tissue engineering.
