Amplifying the manganese scavenging potential of Streptococcus zooepidemicus to reactive oxygen species during production of hyaluronic acid.
- Author:
Mashitah MD
;
Masitah H
;
Ramachandran KB
- Publication Type:Journal Article
- MeSH:
Dose-Response Relationship, Drug;
Free Radical Scavengers/*metabolism;
Hyaluronic Acid/*biosynthesis;
Hydrogen Peroxide/*metabolism;
Magnesium/*pharmacology;
Reactive Oxygen Species/*metabolism;
Streptococcus equi/*drug effects;
Streptococcus equi/metabolism;
Superoxide Dismutase/physiology
- From:
The Medical Journal of Malaysia
2004;59 Suppl B():59-60
- CountryMalaysia
- Language:Malay
-
Abstract:
Streptococcus zooepidemicus (SZ) is an aerotolerant bacteria and its ability to survive under reactive oxidant challenge raises the question of the existence of a defense system. Thus growth, hyaluronic acid (HA) and hydrogen peroxide (H2O2) production by SZ in the presence of increasing concentration of Mn2+ were studied. The results suggested that the tested strain supported growth and HA production in cultures treated with 1 and 10 mM of Mn2+ regardless of H2O2 presence in the medium. This showed that SZ have acquired elaborate defense mechanisms to scavenge oxygen toxicity and thus protect cells from direct and indirect effect of this radical. In contrast, cells treated with 25 mM Mn2+ were sensitive, in which, the HA production was reduced considerably. Thus showing that the oxygen scavenger systems of the cells may be fully saturated at this concentration.
