Strategy for generating tissue-engineered human bone construct.
- Author:
Tan KK
;
Aminuddin BS
;
Tan GH
;
Sabarul Afian M
;
Ng MH
;
Fauziah O
;
Ruszymah BH
- Publication Type:Journal Article
- MeSH:
Bone Regeneration/*physiology;
*Bone Transplantation;
Cell Division/physiology;
Collagen/metabolism;
*Mesenchymal Stem Cell Transplantation;
Organ Culture Techniques;
Periosteum/*cytology;
Tissue Engineering/*methods
- From:
The Medical Journal of Malaysia
2004;59 Suppl B():43-44
- CountryMalaysia
- Language:Malay
-
Abstract:
The strategy used to generate tissue-engineered bone construct, in view of future clinical application is presented here. Osteoprogenitor cells from periosteum of consenting scoliosis patients were isolated. Growth factors viz TGF-B2, bFGF and IGF-1 were used in concert to increase cell proliferation during in vitro cell expansion. Porous tricalcium phosphate (TCP)-hydroxyapatite (HA) scaffold was used as the scaffold to form 3D bone construct. We found that the addition of growth factors, greatly increased cell growth by 2 to 7 fold. TCP/HA proved to be the ideal scaffold for cell attachment and proliferation. Hence, this model will be further carried out on animal trial.
