The use of an in-house biotin-avidin linked immunosorbent assay to detect Aspergillus antigens in sera of immunocompromised patients.
- Author:
S Abdul Samad
;
H Yusoff
;
S A Fadilah
- Publication Type:Journal Article
- MeSH:
Antigens;
assay;
Aspergillus;
Immunocompromised Host;
Biotin
- From:
The Medical journal of Malaysia
2001;56(1):32-8
- CountryMalaysia
- Language:Malay
-
Abstract:
A biotin-avidin-linked immunosorbent assay was developed to detect Aspergillus antigens in sera of immunocompromised patients. The assay was based on a double antibody sandwich ELISA using polyclonal antibodies raised against water-soluble antigens of Aspergillus fumigatus. Aspergillus antigens were positive in sera of 9 of 16 (56%) patients who were studied prospectively and in 13 of 73 (19%) patients studied retrospectively. The 9 prospectively studied patients who were antigen positive were febrile neutropenic hematological malignancy patients who exhibited a high risk of acquiring invasive aspergillosis.
