Effect of Traditional Chinese Medicine Complex for diabetes (TCM-D™) on experimentally induced diabetic mice
- Author:
Donald Koh Fook Chen
;
Joon Wah Mak
;
Soo Shen Ooi
;
Kok Fee Mak
;
Kwai Hoe Chong
- Publication Type:Journal Article
- Keywords:
Traditional Chinese Medicine;
experimental diabetes in mice;
Type 1 diabetes mellitus, Trichosanthes kirilowii Maxim;
Paeonia lactiflora Pall;
Glycyrrhiza uranlensis Fisch;
Panax ginseng Meyer (red)
- From:International e-Journal of Science, Medicine and Education
2013;7(1):4-9
- CountryMalaysia
- Language:English
-
Abstract:
Background: We previously evaluated the biochemical
changes induced by the local product TCM for
diabetes (TCM-D™) on blood glucose levels and other
biochemical changes in normal mice fed orally with the
recommended human dose (30 ml/kg daily) and ten
times this dose for eight weeks. TCM-D™ is an aqueous
extract of the roots of Trichosanthes kirilowii Maxim,
Paeonia lactiflora Pall, Glycyrrhiza uranlensis Fisch. and
Panax ginseng Meyer (red) combined at the dry weight
proportions of 36%, 28%, 18% and 18% respectively.
The study showed that at these dosages the blood
glucose levels as well as the body weights in treated mice
were significantly reduced when compared with pretreatment
values and control animals. The present study
evaluated the effect of the extract in a mouse model of
Type 1 diabetes mellitus.
Methods: TCM-D™ extract was prepared as a 10x
concentrate and given orally at 0.3 ml/100 g and
1.5 ml/100 g to mice which were experimentally
induced diabetic with intraperitoneal injections of
streptozotocin (5 mg/100g) in sodium citrate (pH 4.5).
Control diabetic mice were dosed with extract diluent
(distilled water).
Results: At the doses studied the compound did not
show any significant lowering of the glucose levels in a
mouse model of Type 1 diabetes. There were significant
increases in the alanine aminotransferase (ALT) and
creatinine levels which were most likely due to the
treatment with the compound. There were no significant
changes in the aspartate aminotransferase (AST) and
blood urea levels due to the treatment. Neither was
there any significant effect on the weight of the treated
animals due to the treatment.
Conclusions: It is concluded that TCM-D™ did not
have any significant blood glucose lowering effect on
streptozotocin induced diabetic mice when fed orally at
1-5 times the recommended human dose. Further work
is needed to determine if the extract has any significant
effect in a mouse model with Type 2 diabetes mellitus.
- Full text:W020151005526835743925.pdf
