Searching for Appropriate Statistical Parameters for Validation of Mitochondrial DNA Database.
10.7580/kjlm.2014.38.2.59
- Author:
Chong Min CHOUNG
;
Ji Hyun LEE
;
Sohee CHO
;
Soong Deok LEE
- Publication Type:Original Article
- Keywords:
mtDNA DB;
Statistical parameter;
Coverage;
Phylogeny;
Saturation curve
- MeSH:
DNA;
DNA, Mitochondrial*;
Haplotypes;
Korea;
Phylogeny
- From:Korean Journal of Legal Medicine
2014;38(2):59-65
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Recently, studies on mitochondrial DNA (mtDNA) have increased rapidly. Conventional parameters, such as diversity index, pairwise comparison, are used to interpret and validate data on autosomal DNA; however, the use of these parameters to validate data from mitochondrial DNA databases (mtDNA DBs) needs to be verified because of the different transmission patterns of mtDNA. This study was done to verify the use of these conventional parameters and to test the "coverage concept" for a new parameter. The mtDNA DB is not very big; however, it is necessary to check how the change in parameters corresponds to the DB size. For this, we artificially rearranged a Korean DB into several small sub-DBs of variable sizes. The results show that the diversity in nucleotide variations and the different haplotype numbers do not vary as the size of DB increases. However, the "coverage" changed a lot. The coverage increased from 0.113 in a DB of 100 people to 0.260 in a DB of 653 people. Additionally, using the "coverage concept", we predicted how the total number of haplotypes changed with variations in the sub-DB size and compared the predicted result with final result. In conclusion, "coverage", in addition to conventional statistical parameters, can be used to check the usability of an mtDNA DB. Finally, we tried to predict the size of the whole mtDNA number in Korea using "saturation concept".
