Ability of Acanthamoeba Cyst to Excyst at Different Temperatures
- Author:
Nurul Fariza Rossle
;
Anisah Nordin
;
Yusof Suboh
;
Noraina Ab Rahim
;
Mohamed Kamal Abd Ghani
- Publication Type:Journal Article
- Keywords:
Thermotolerance, Acanthamoeba cyst, Excystation
- From:Malaysian Journal of Health Sciences
2011;9(1):41-43
- CountryMalaysia
- Language:English
-
Abstract:
This study was carried out to observe thermotolerance ability of Acanthamoeba spp. A total of 32 Acanthamoeba spp. isolates obtained from water taps, sinks, swimming pools and sea water were used. Trophozoites of Acanthamoeba spp. were inoculated onto non-nutrient agar (NNA) seeded with heat-killed Escherichia coli using aseptic technique and incubated for 14 days at 30°C to obtain the cyst. The cysts were subcultured onto new agar plates for thermotolerance test at 37°C and 42°C. The plates were observed until 96 hours after incubation for excystation of Acanthamoeba before being declared negative. Overall, 81.25% of samples were able to excyst at 37°C while 37.5% were able to excyst at
42°C. Thermotolerant Acanthamoeba is associated with high pathogenicity potential.
- Full text:W020150622402599615742.pdf
