Characterization of tumorspheres generated from nasopharyngeal carcinoma cell line, TW06 and chemoresistance to docetaxel and oxaliplatin
- Author:
Iris Goh Wen Li
;
Yip Wai Kien
;
Seow Heng Fong
- Publication Type:Journal Article
- Keywords:
Tumorspheres;
Cancer stem cells;
Nasopharyngeal carcinoma;
Chemoresistance
- MeSH:
Neoplastic Stem Cells;
Nasopharyngeal carcinoma
- From:Malaysian Journal of Medicine and Health Sciences
2015;11(2):9-20
- CountryMalaysia
- Language:English
-
Abstract:
In this study, tumorspheres were generated from TW06 nasopharyngeal carcinoma cell line and
examined their expression of putative cancer stem-like cell surface markers and drug sensitivity. The
rate of tumorsphere expansion from dissociated late passage TW06 tumorspheres (≥ passage 15) was
higher than that from parental cells and dissociated 10-day-old (passage 0) tumorspheres. The expression
of CD24 surface marker was lost in the generation of tumorspheres and the loss was reversible after
differentiating the tumorspheres in monolayer culture conditions. Drug sensitivity assay showed that late
passage tumorspheres were resistant to docetaxel and oxaliplatin treatment. Our data suggest that serially
passaged tumorspheres possess the characteristics of CSCs that render them a suitable preclinical in
vitro model for evaluating anticancer drug efficacy and elucidating the underlying mechanisms of drug
resistance.
- Full text:P020151209531853609655.pdf
