Total Phenolic Content, Antioxidant and Cytotoxic Activity of Cocoa (Theobroma cacao L.) Polyphenols Extracts on Cancer Cell Lines
- Author:
Hazirah AR
;
Zainal B
;
Abdah MA
- Publication Type:Journal Article
- Keywords:
Cocoa polyphenols extract, DPPH, FRAP, MTT assay,TPC
- From:
Malaysian Journal of Nutrition
2013;19(2):223-232
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Cancer chemopreventive agents from natural sources have been
actively investigated over the years to seek prevention against cancer. In this
study, cocoa polyphenols extract (CPE) was examined to explore its antioxidant
and cytotoxicity activities. Methods: CPE was analysed for total phenolic content
(TPC) and antioxidant activity (DPPH radical scavenging activity and FRAP
ferric-reducing antioxidant power assays). In vitro cytotoxicity effect of CPE
against HepG2, HT-29, HeLa, MCF-7, MDA-MB-231 and WRL-68 cell lines after
48 h exposure was measured by MTT assay. Results: The study showed that CPE
had higher total phenolic content (13560.0±420.1 mg GAE/100g dry weight of
sample) than vitamin E (p<0.05). CPE exhibited strong antioxidant activity
comparable with ascorbic acid in both DPPH (IC50 = 14.73±1.47 g/ml) and FRAP
(2130.33±2.33 μM of FE/1 mg of dry weight of sample). The cytotoxicity study
showed that CPE exhibited the highest cytotoxicity effect against MCF-7 with
lowest IC50 value (3.00±0.29 mg/ml) compared to other cancer cell lines after 48
h treatment (p<0.05). Conclusion: Our results indicate that CPE demonstrated
high total phenolic content, free radical scavenging activity, ferric reducing
ability and cytotoxicity activity towards HepG2, HT-29, HeLa, A549, MDA-MB-
231 and MCF-7 cancer cell lines. Further isolation of bioactive constituents from
CPE should be done to characterise its potential chemopreventive activity as
well as to elucidate the mechanism of cancer cell death induced by CPE.
- Full text:W020150716572031240212.pdf
