A Study of Effect of Diabetic Sera on in Vitro Growth of Candida albicans.
- Author:
Kee Chan MOON
;
Hong Shik KIM
- Publication Type:In Vitro ; Original Article
- MeSH:
Adult;
Arthrodermataceae;
Blood Glucose;
Candida albicans*;
Candida*;
Humans;
Iron;
Spectrophotometry;
Transferrin
- From:Korean Journal of Dermatology
1979;17(4):245-251
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
It has been well known that human sera exert an inhibitory effect on the growth of dermatophytes and candida. Several different inhibitory serum factors have been isolated and investigated by some workers. In clinical aspects, the patients with diabetes are prone to derrnatophyte and candida infections. The authors attempted to investigate whether diabetic sera would have any difference in inhibitory effect on in vitro candidial growth compared with norma1 sera, Fifty and 30 serum samples were obtained from patients with diabetes and bealtby adults respectively, Aliquots of candida albicans were mcubated in the test tubes containing nutrient kiroth with addition of eacb serum. Iron was also added to saturate transferrin in the serum. The degree of candidial growth was determined by the change of percent transmission on spectrophotometry of the candida-inoculated nutrient broth in the test tubes during 24-hour incubation. The results were as followings: 1. The sera from both patients with diabetes and healthy adults were found to retain the inhibitory effect on in vitro candidial growth, though the transferrin, a known serum inhibitor, was removed by saturation with iron. 2. The inhibitory effect of diabetic sera was weaker than that of normal sera. 3. The degree of the inhibitory effect of each diabetic serum was not correlated with corresponding serum glucose level.
