Functional imaging and pathology in brain of interictal cats kindled by pentylenetetrazol
- Author:
Gonglu Liu
;
Kaiyan Wang
;
Chuanzhen Lv
;
Jun Zhao
;
Xingdang Liu
;
Chuantao Zuo
- Publication Type:Journal Article
- From:Neurology Asia
2013;18(1):9-15
- CountryMalaysia
- Language:English
-
Abstract:
Objective: To investigate the electroencephalogram (EEG) characteristics, cerebral blood fl ow, glucose
metabolism, and pathological characteristics in cats kindled by pentylenetetrazol (PTZ). Methods: Ten
adult male cats received intramuscular injections of PTZ at a sub-convulsive dose of 25 mg/kg once
daily. Cats were considered to be kindled when a class V or above seizure was observed. Subsequently,
scalp EEG, 99mTc-ECD-single-photon emission computed tomography (SPECT), 18FDG-positron
emission tomography (PET), and Cresyl violet staining were employed to evaluate brain activity,
cerebral blood fl ow, glucose metabolism, and pathological characteristics representing epileptic foci
during interictal stages. Results: The EEG data revealed bilateral dissymmetry paroxysmal activity in
the form of δ and θ waves, as well as paroxysmal spikes and sharp waves instead of symmetric highamplitude
rhythm of 8-12 Hz in cats kindled by PTZ compared to controls. In addition, low blood
perfusion and low glucose metabolism unilaterally in the temporal lobe were observed in PTZ-kindled
cats, in contrast to the bilateral symmetrical blood perfusion and high glucose metabolism observed in
control cats. Pathological examinations showed a thickening of white matter in the ipsilateral temporal
region and a thinning of gray matter in PTZ-kindled cats. Microscopically, we observed a structure
disturbance of hippocampal CA3 area in PTZ-kindled cats.
Conclusion: Epileptic foci of PTZ-kindled cats were localized to the unilateral temporal lobe, in a
manner highly similar to the pathophysiology of human temporal lobe epilepsy.
- Full text:P020150703571264545036.pdf
