Myelotomy suppresses autophagic marker LC3-II expression and elevates mTORC1 expression and improves neurological function recovery in rats with gical function recovery in rats with
- Author:
Degang Yang
;
Jianjun Li
;
Rui Gu
;
Anming Hu
;
Mingliang Yang
;
Liangjie Du
;
Xin Zhang
;
Wei Sun
;
Feng Gao
;
Yingying Wu
;
Jiangen He
;
Yutong Feng
;
Hongyu Chu
- Publication Type:Journal Article
- From:Neurology Asia
2013;18(4):401-407
- CountryMalaysia
- Language:English
-
Abstract:
Although previous studies have shown functional efficacy of myelotomy for the treatment of spinal
cord injury (SCI), the underlying mechanism remained unknown. This study aimed to determine
the relationship between myelotomy-mediated neuroprotection and autophagy following SCI by
evaluating the expression of microtubule-associated protein light chain 3 (LC3-II) and mammalian
target of rapamycin complex 1 (mTORC1). Ninety-nine adult female rats were randomly assigned
to either sham-operated group (SG), model group (MG), or 24 h-myelotomy group (MTG). SCI at
T10 was induced with a New York University impactor, and myelotomy was performed 24 h after
SCI. Functional recovery was evaluated via the open-field test. The protein expression of LC3-II
was analyzed by Western blot, and the mRNA expression of LC3-II and mTORC1 were detected by
real-time quantitative reverse transcriptase polymerase chain reaction. Rats in the MTG exhibited
significantly better performance in the hind limbs compared to those in the MG on day seven and
fourteen post-injury. Myelotomy suppressed the protein and mRNA expression of LC3-II on day three,
seven and fourteen post-injury and increased the mRNA expression of mTORC1 in the MTG on day
three and seven post-injury. The LC3-II protein expression was significantly and negatively correlated
with BBB scores at day seven and fourteen post-injury. These results showed that myelotomy-induced
neuroprotection in a rat model of SCI was likely mediated by inhibition of autophagy by activation
of the mTORC1 signaling pathway
- Full text:P020150702604638541368.pdf
