Genomic Diversity of Cholera Outbreak Strains in East Malaysia
- Author:
Lesley Maurice Bilung
;
Yong Sy Fuh
;
Velnetti Linang
;
Adom Benjamin
;
Micky Vincent
;
Kasing Apun
;
Samuel Lihan
;
Chien Su Lin
- Publication Type:Journal Article
- Keywords:
Vibrio cholerae;
Ctx gene;
ERIC-PCR;
BOX-PCR;
Genetic fingerprinting
- MeSH:
Cholera
- From:Malaysian Journal of Medicine and Health Sciences
2014;10(2):19-26
- CountryMalaysia
- Language:English
-
Abstract:
Thirty one Vibrio cholera isolates recovered from cholera outbreak in Bintulu, Sarawak (Malaysia) were
detected with the presence of ctx gene by using specific PCR. These isolates were further characterized
and differentiated by using the Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) and
BOX-PCR to determine their genomic fingerprints. The specific PCR result confirmed the identities
of 27 isolates out of 31 as pathogenic V. cholerae. The ERIC-PCR generated several genetic profiles
consisting of 4-6 bands with sizes in the range of 100 to 600 bp, while the BOX-PCR produced profiles
numbering 2-7 bands in the sizes between 200 to 1000 bp. Based on the dendrogram generated from the
DNA fingerprinting profiles (ERIC-PCR and BOX-PCR), all of the isolates can be divided into 2 main
clusters that is further divided into 2 sub-clusters. The low genetic diversity of the isolates indicated the
outbreak of V. cholerae in the study area was due to the contamination from a single or few sources of
V. cholerae.
- Full text:P020150604511253739574.pdf
