Immature Reticulocyte Fraction in Guiding Stem Cell Harvest in Autologous Peripheral Blood Stem Cell Transplant
- Author:
M.N. Sabariah
;
C.F. Leong
;
S.K. Cheong
- Publication Type:Journal Article
- Keywords:
Autologous PBSCT;
PB CD34+ count;
immature reticulocyte fraction
- MeSH:
Peripheral Blood Stem Cell Transplantation;
Hematopoietic Stem Cells
- From:Malaysian Journal of Medicine and Health Sciences
2014;10(1):1-6
- CountryMalaysia
- Language:English
-
Abstract:
Peripheral blood (PB) CD34+ cells enumeration is currently the most reliable method to guide the
timing of stem cell harvest. However, its usage is restricted by being technically challenging, costly,
and time-consuming. Immature reticulocyte fraction (IRF) determination, which is simpler and cheaper
and has a faster turn-around time, has been proposed for a similar purpose. The purpose of this study
is to evaluate the value of IRF in guiding stem cell harvest and examine the correlation between IRF
and PB CD34+ cells count. Daily pre-harvest tests, i.e. PB CD34+ cells and IRF from 21 patients
scheduled for autologous PBSC transplant were assessed. Stem cells harvests were commenced when
the PB CD34+ cell count were more than 10 cell/ul. A total of 205 pre-harvest tests were analysed.
Following stem cell mobilisations, both the IRF and PB CD 34+ cell counts rose with a variable pattern.
In this study, we observed that the IRF peaks preceded the PB CD34+ count by 2 days. On the day
of stem cell harvest, all the peak IRF values were >0.3. The PB CD34+ cell counts correlated with
the harvested stem cell yield, whereby r2 = 0.77, p < 0.021. In autologous stem cell mobilisation,
we believe that IRF is a useful screening tool to predict the rise of the PB CD34+ cell counts as it is
a simple, fast and less costly. An IRF of > 0.3 may be used as a cut-off value for the initiation of PB
CD34+ quantifi cation prior to stem cell harvest.
- Full text:P020150604475921546096.pdf
