Genotoxic evaluation of synthetic hydroxyapatite using mammalian bone marrow chromosome aberration test
- Author:
Thirumulu Ponnuraj Kannana
;
Nik Ahmad Shah Nik Lah
;
Azlina Ahmad
;
Siti Fatimah Ramli
;
Narazah Mohd Yusoff
;
Ab Rani Samsudin
- Publication Type:Journal Article
- Keywords:
Chromosome aberration;
cytotoxicity;
genotoxicity;
hydroxyapatite;
mice
- MeSH:
Chromosome Aberrations;
Durapatite
- From:Archives of Orofacial Sciences
2014;9(1):10-16
- CountryMalaysia
- Language:English
-
Abstract:
Some of the beneficial biocompatible properties of hydroxyapatite [Ca10(PO4)6(OH)2]; the major component
and an essential ingredient of normal bone and teeth, are that it is rapidly integrated into the human body and will bond
to bone forming indistinguishable unions. But, before new materials are approved for medical use, mutagenesis
systems to exclude cytotoxic, mutagenic or carcinogenic properties are applied worldwide. This study aimed to detect
any chromosomal aberrations induced by the synthetic hydroxyapatite granules [Manufactured by Universiti Sains
Malaysia, (USM) Penang, Malaysia] in the bone marrow cells of mice. The mitotic indices of the groups treated with
synthetic hydroxyapatite granules did not show any significant difference as compared to the negative control group
treated with distilled water. Also the groups of mice treated with synthetic hydroxyapatite granules and distilled water
did not induce significant change in chromosome aberrations as compared to the positive control group treated with
Mitomycin C. The mitotic indices and chromosomal analyses indicate that under the present test conditions, synthetic
hydroxyapatite granules (manufactured by USM) are non cytotoxic and do not induce chromosome aberrations in the
bone marrow cells of mice.
- Full text:P020141126458079015644.pdf
