Correlation of Nuclear Morphometry and AgNOR Score with Radiation Response in Squamous Cell Cancers of the Head and Neck: A Preliminary Study
- Author:
Biswa Mohan Biswal
;
Nor Hayati Othman
- Publication Type:Journal Article
- Keywords:
AgNORs, fine-needle aspiration, squamous cell cancer, radio-sensitivity, radiotherapy, radiology, medical sciences
- From:Malaysian Journal of Medical Sciences
2010;17(3):19-26
- CountryMalaysia
- Language:English
-
Abstract:
Background: Prediction of radiation response before the completion of the radiotherapy
schedule is challenging. Information about radiation response could help oncologist to choose the
appropriate combination and sequence of therapies in the multidisciplinary management of cancer.
Methods: The study involved 26 patients with squamous cell cancers of the head and neck
region who received radiotherapy to a dose of 30 Gy in 10 fractions over a 2-week period as part of
a split-course technique. Fine-needle aspiration cytology was performed on day 1 and day 5 of the
schedule. The silver staining of the nuclear organiser region (AgNOR) and nuclear morphometric
study were done on both days.
Results: The median age of the patients was 44 years old. The primary tumours were
distributed in the nasopharynx (n = 11), larynx and hypopharynx (n = 5), metastatic node (n = 4), and
miscellaneous tumours were found in the head and neck sub sites (n = 6). The mean initial AgNOR
score was 3.0, range 1.2–7.0. The median of nuclear and nucleolar diameters were 11.07 μm, range
7.70–16.6 μm, and 2.92 μm, range 1.09–11.66 μm, respectively. Patients with a pre-radiotherapy
AgNOR score of greater than 2.5 were associated with disease progression and metastasis. However,
the increased of nuclear diameter on day 5 compared with baseline predicted a good radiation
response in patients (P = 0.016).
Conclusion: Intra-radiotherapy nuclear morphometry combined with baseline AgNOR score
could be a simple and useful tool for the prediction of radiation response in head and neck cancers.
- Full text:W020151022443225037005.pdf
